In image cytometry, each cell traverses a linear transparent microchannel. As a specified region within the channel is passed, an image or series of images is recorded. The images are processed in real time or later, and a list of biomarkers per cell is extracted. Bright-field imaging extracts markers including cell size, shape, opacity, color, elasticity, granularity and insights from stained cellular components. Fluorescence imaging characterizes the amount and position of key biomolecular species within the cell, including DNA and actin. Due to the low light nature of fluorescence relative to the necessary camera integration times, this study is limited to bright-field imaging, which is common among researchers pushing the limits of high-throughput image cytometry.  Our infographic on imaging cytometry helps to explain how high-speed cameras enhance this unique research method. 

Image Cytometry